Coding

Part:BBa_K3971004:Design

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-08-09)


sps - Sucrose-phosphate synthase gene in Cyanobacteria.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1676
    Illegal PstI site found at 1714
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1676
    Illegal NheI site found at 1869
    Illegal PstI site found at 1714
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1676
    Illegal XhoI site found at 1453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1676
    Illegal PstI site found at 1714
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1676
    Illegal PstI site found at 1714
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

This gene sequence was present in a strain of Synechococcus elongatus UTEX 2973 generously donated to us by the Pakrasi Lab at Washington University.

References